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Form: buffered aqueous solution concentration 10,000 – 50,000 U/mg
Aug 29, 2017. A mutant T7 RNA polymerase with a nucleotide substitution error rate at least 20- fold greater than that of the wild-type was selected.
Feb 23, 2001. Abstract. Viruses replicate their genomes at exceptionally high mutation rates. Their offspring evolve rapidly and therefore, are able to evade.
12. Non-PCR Ampliﬁcation 185 1989; Guatelli et al., 1990). This ampliﬁcation strategy depends on each RNA amplicon serving as a template for additional rounds of.
DNA primase is an enzyme involved in the replication of DNA and is a type of DNA polymerase. Primase catalyzes the synthesis of a short RNA (or DNA in.
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4 Department of Pathology, University of Texas Medical Branch at Galveston, Galveston, TX 77555, USA. 5 Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Nucleotide selection is crucial for transcription fidelity control, in particular, for viral T7 RNA polymerase (RNAP.
What is the error rate of RNA transcription?.(2014) – Quora – The error rate of RNA synthesis is of the order of one mistake per 10^4 or 10^5. of enzyme called RNA polymerase moves along the DNA template, reading the.
Feb 24, 2012. Results: T7 RNA polymerases back up by DPCs and generate. relatively high transcriptional fidelity (error rates of 10−4–10−5) (27,–,29), and.
Double Nicking by RNA-Guided CRISPR Cas9 for. – Targeted genome editing technologies have enabled a broad range of research and medical applications. The Cas9 nuclease from the microbial CRISPR-Cas system is
The authors evolved T7 RNA polymerase variants with new promoter. Although the authors attempted to increase the mutation rate by using a plasmid encoding the error-prone DNA polymerase V as well as a dominant-negative form of.
T7 RNA polymerase – Wikipedia – T7 RNA Polymerase is an RNA polymerase from the T7 bacteriophage that catalyzes the formation of RNA. It has a very low error rate. T7 polymerase has a molecular.
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T7 RNA Polymerase is an RNA polymerase from the T7 bacteriophage that catalyzes the. It has a very low error rate. T7 polymerase has a molecular weight of.